"One of the biggest advantages of the Patchliner® for our lab is that it enables students and fellows without formal training in patch clamp electrophysiology to begin generating meaningful data almost immediately. Instead of spending a couple of frustrating months learning the motor skills and hand-eye coordination necessary for conventional patch clamping with a microscope and micromanipulator, they spend that time designing and executing experiments, generating, evaluating and interpreting data and moving the project forward. The Patchliner®also enables experimental flexibility that would be very difficult or impossible with conventional patch clamp rigs. For example, a talented undergraduate in the lab just finished up a series of intracellular drug application experiments. These studies would be difficult for the seasoned electrophysiologist and nearly impossible for an undergraduate student. However, the Patchliner® allowed them to be completed in a very short period of time.
Another major advantage is that the Patchliner® enables us to rapidly confirm or exclude hits from our primary fluorescence-based high-throughput screen using gold-standard electrophysiological methods. And the quality of the recordings rivals that of a conventional patch clamp rig. In fact, our first observations of pore "knock-off" of a new ROMK inhibitory small-molecule came from the Patchliner®. These early observations from high-quality Patchliner® recordings provided a clear direction for subsequent mutagenesis work aimed at defining the binding site of this molecule within the cytoplasmic channel pore."
Jerod S. Denton, Ph.D.
Assistant Professor of Anesthesiology and Pharmacology
Vanderbilt University Medical Center
Nashville, TN, USA
"In 2006 we purchased two of Nanion"s very first Patchliner® systems for our laboratories at the University of Leeds. We have been working with both systems very successfully for nearly four years now which is reflected in our recent high quality publications.
We operate both Patchliner® systems on a daily basis, serving five different laboratories within the Faculty of Biological Sciences. Due to the diversity between the five groups, our Patchliners® have been thoroughly put through their paces. This means that we have tested a large variety of different cells and a vast array of channel types, both endogenously and exogenously expressed. For example, we have investigated endogenous transient receptor potential (TRP) channels in human vascular smooth muscle cells and human synoviocytes. In addition, we have studied endogenous ion channels in primary astrocytes, human lymphoblasts and neutrophils, to mention a few.
The high data output has resulted in the production of an enormous amount of extremely good quality data using both native and primary mammalian cells. Our ability to make successful recordings from primary cells using the Patchliner® is an extraordinary accomplishment, especially because the primary cell types that we work with are generally very difficult to record from using conventional patch clamp. Another impressive feature of this platform is its ability to perform routine intracellular perfusion which has enabled studies which might not have previously been attempted. We have also included our new technology as part of our annual "Leeds Ion Channel Workshop" where it is well received.
The Patchliner® has turned out to be a show case project for us and has opened up new opportunities which I believe will play important roles in the future successes of research projects here in Leeds."
Dr. Carol Milligan
Research Fellow, Faculty of Biological Science, Leeds University, Leeds, UK
Further User Statements
"Xention is a company specialising in ion channel drug discovery and development. We have invested in Patchliners® because their flexibility and adaptability allow us to utilise this technology to meet the differing needs of our various screening projects. The targets we study require high quality electrophysiological recordings. We have found that the Patchliner® can deliver such output with a high success rate for a range of different ion channels and heterologous cell lines. Nanion has been extremely helpful in customising the software to fit our screening requirements. I would happily recommend the Patchliner® to anyone who needs reliable patch clamp instrumentation for their ion channel screening purposes.”
Dr. Marc Rogers, Principal Scientist, Xention Ltd., Pampisford/Cambridge, UK
"To invest in the Patchliner® was a straightforward choice for the Vanderbilt Screening Center. This instrument combines a set of features that was particularly important to us. (...) Unlike other planar patch clamp devices we considered, the Patchliner® gave us full access to the voltage clamp, current clamp, standard whole cell, cell attached, and perforated patch configurations combined with a facile ability to exchange the internal solution. (...) In a short time the Patchliner® has already begun to expand Vanderbilt investigators’ horizons regarding what they can accomplish with electrophysiology."
Dr. David Weaver, Research Associate Professor of Pharmacology, Director of the Chemical Biology"s High-Throughput Screening Facility, Vanderbilt University, Nashville, TN, USA
"In Evotec"s continued effort to provide the most valuable services to our customers and increase our capabilites in our ion channel platform, we have invested in multiple Patchliner® instruments. These have allowed us to complement our existing patch clamp capabilities and substantially increase our throughput in a number of electrophysiology and screening projects, including hERG screening. The Patchliners® provide us with high quality data, are easy to operate with minimal assay development time, and we are particularly happy with the first class support Evotec receives from Nanion."
Dr. Clemens Möller, Team Leader Electrophysiology, Evotec AG, Hamburg, Germany
"The Port-a-Patch® is a state-of-the-art automated patch clamp platform that allows trainees at Hydra without prior experience in electrophysiology to quickly learn how to generate high quality patch clamp recordings. It speeds up the teaching process substantially and gives us an easy-to-use platform for efficient, accurate screening of ion channel active compounds."
Dr. Chris Fanger, Director of Lead Discovery, Hydra Biosciences, Boston, MA, USA
"The Port-a-Patch® enables students and young researchers lacking formal training in electrophysiology to rapidly generate research grade data. Within the courses given at the Institute for Biomolecular Systems and Bioelectronics, the students gain hands-on experience in basic membrane biophysics and electrophysiology, which is of great value for their education."
Professor Friedrich Simmel, Technical University of Munich, Munich, Germany
"We are using the Vesicle Prep Pro® and Port-a-Patch® in our laboratory to study recombinant channel proteins reconstituted in planar bilayers. The system is easy to use and it makes experiments very efficient. Besides the great equipment, Nanion, with their widespread expertise, is also providing excellent continuous support, helping with methodology optimization and problem solving."
Dr. Colin Taylor, Professor of Cellular Pharmacology, Department of Pharmacology, University of Cambridge, Cambridge, UK
"Our planar lipid bilayers are obtained from GUVs prepared with the Nanion Vesicle Prep Pro® setup. Together with the Port-a-Patch®, Nanion´s state-of-the-art automated patch clamp platform, the efficiency of characterization of pore-forming peptides (and other ionophores from natural sources) in artificial bilayers has greatly increased. Both systems are straightforward and easy to use, allowing high quality, low noise, single channel recordings."
Marisa Rangel, PhD, Researcher, University of Sao Paulo, Butantan Institute, Sao Paulo, Brazil
"The Vesicle Prep Pro® has been of great utility in our Bioelectronics research, allowing students and researchers with limited electrophysiology background to easily form giant unilamellar vesicles (GUVs). The electronic control lets us alter the size and density of the GUVs, and the transparent chamber allows for excellent visualization of the electro-swelling process."
Eric Stava, PhD, Research Assistant, University of Wisconsin-Madison, WI, USA